| 质粒名称: | 1432 pcDNA3 flag MAGI1c,pCDNA3-FLAG-MAGI1c-m |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 10714 |
| 存储实验室: | William Sellers |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5446 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MAGI-1,MAGI1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 6000 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | flag（插入N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | 1432 pcDNA3 flag MAGI1c was a gift from William Sellers (Addgene plasmid # 10714 ; http://n2t.net/addgene:10714 ; RRID:Addgene_10714)T7 |
| 参考文献部分: | Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR. J Biol Chem. 2001 Dec 28. 276(52):48627-30. 10.1074/jbc.C100556200 PubMed 11707428 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 1432 pcDNA3 flag MAGI1c 9835 bp ds-DNA circular SYN 07-MAY-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 1432 pcDNA3 flag MAGI1c
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9835)
AUTHORS Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers
WR
TITLE Phosphorylation of the PTEN tail acts as an inhibitory switch by
preventing its recruitment into a protein complex.
JOURNAL J Biol Chem. 2001 Dec 28. 276(52):48627-30.
PUBMED 11707428
REFERENCE 2 (bases 1 to 9835)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9835
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 173..552
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 553..756
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 707..727
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 801..820
/label=T7
/note="T7 promoter, forward primer"
promoter 801..819
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 845..868
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 872..895
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(5326..5344)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5327..5344)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5364..5381)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5370..5594
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5640..6068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5727..5746)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5937..5958
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6077..6097)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6082..6411
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6262..6397
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6324..6343
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6478..7272
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6532..6551)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7142..7161
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7446..7567
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7483..7502)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7537..7556
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7616..7632)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7616..7632)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7629..7651)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7640..7656
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7664..7694)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7709..7730
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7847..7864)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8018..8606)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8098..8117)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8777..9637)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9400..9419
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9638..9742)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9817..1)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
61 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
121 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
721 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
781 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc ttctcatcat
841 gggcgactat aaggatgacg atgacaaggg cgattacaaa gacgatgacg ataaatcgaa
901 agtgatccag aagaagaacc actggactgg ccgcgttcac gagtgcaccg tgaagcgggg
961 accccagggc gagctggggg tgacggtcct ggggggcgcg gagcatgggg agtttccgta
1021 cgtgggggcg gtggcggcgg ccgaggcggc ggggcttccc ggcggtggcg aggggccgaa
1081 gctggccgaa ggtgagctgc tgctggaggt gcagggggtc cgggtgtccg gcttgccccg
1141 ctatgacgtg ctgggagtca tcgacagctg caaggaggcc gtcaccttca aagccgtcag
1201 acaaggagga aggctcaaca aggacctacg acatttcctc aaccaacggt tccagaaggg
1261 gtctccagat catgagctcc agcagaccat aagggacaac ctctaccgcc atgctgtgcc
1321 ttgcacaacc cggtctccca gagaaggaga agtgcctggc gtggattaca gctttctgac
1381 tgtgaaggag ttcttggacc tcgagcagag cgggaccctg ttggaagtcg gcacctatga
1441 aggaaactat tatgggacac ccaaacctcc cagccagcca gtcagtggga aagtgatcac
1501 gacggatgcc ttgcacagcc tgcagtctgg ctccaaacag tcgaccccta agcgaacaaa
1561 gtcctacaat gatatgcaaa atgctggcat agtccacccg gagaatgagg aggaggagga
1621 tgtccctgaa atgaacagta gctttacagc cgactctgga gaccaggacg agcacactct
1681 ccaagaagca acgctcccac ctgtgaatag tagcatcctc gctgctccca tcacggaccc
1741 ttctcagaag ttccctcagt acctacctct ttctgcagag gataatttag gtcctctacc
1801 tgaaaactgg gagatggcct atactgaaaa tggagaagtc tatttcatag accacaacac
1861 gaaaacaaca tcatggttag accctcggtg cctgaacaaa cagcagaagc ctctggaaga
1921 atgtgaagat gatgagttgc ctgctggctg ggaaaagatt gaagaccctg tctacggtgt
1981 ctactatgta gaccacatca acaggaagac gcaatatgaa aacccagtcc tagaagccaa
2041 acggaagaaa cagcttgaac agcagcagca acagcagcag cctcagccac cgcagccaga
2101 agagtggaca gaggatcatg catctgttgt gcctcctgtt gctccttccc atcccccgag
2161 caatccggag ccagccaggg aaactccact tcagggcaaa ccttttttta caagaaaccc
2221 ctctgagctg aaaggcaagt tcattcacac gaagctacgg aaaagcagcc gaggctttgg
2281 cttcacggtg gttggaggag acgagcctga tgagttcctg cagatcaaga gcctcgtcct
2341 cgatggtcct gccgcactgg atggcaagat ggagacaggg gatgtaattg tgagtgtgaa
2401 tgacacctgt gttttgggac acacacatgc tcaagttgtg aaaatcttcc agtccattcc
2461 cattggtgcc agtgtggacc ttgaactctg cagaggttat ccattgcctt ttgacccgga
2521 tgaccctaat acaagtttag tgacctcggt ggccattttg gacaaagaac caattattgt
2581 aaatggacaa gagacctacg attcaccagc gagccacagt agtaaaacag gcaaagtcag
2641 cagcatgaag gacgccaggc caagcagccc tgctgatgtg gcttccaaca gctctcatgg
2701 ttaccccaac gacacagtct ccttggcttc ctccatagcc acccagccag agctaataac
2761 tgttcacata gtcaaagggc caatgggatt tggctttacg atcgcagaca gtcccggtgg
2821 gggtggccaa agagtgaaac agattgttga cagtccacgc tgcagaggcc tcaaagaagg
2881 ggatcttatc gtggaggtga ataagaagaa cgtgcaggcc ctgacgcaca atcaagtcgt
2941 ggatatgctg attgaatgtc ccaagggaag tgaggtcaca ctgttggtgc agcgaggagg
3001 gctaccagtt cccaagaaga gcccaaagtc gccactggag aggaaagaca gccagaatag
3061 ctcccagcac agcgtctcca gccaccggag cctgcacact gcgtccccga gccacggcat
3121 acaggtgctc cctgagtacc tacctgcaga cgcccctgct ccagatcaga ccgacagctc
3181 tgggcagaaa aagccagatc cttttaaaat ctgggcccag tccaggagca tgtatgaaaa
3241 ccgacttcca gattaccagg aacaggacat cttcctctgg agaaaagaaa ccggatttgg
3301 atttaggatt ctgggtggaa atgaaccagg ggaacccatt tatatcggtc acatcgtacc
3361 gctgggtgct gctgacacag acggccgcct gaggtctgga gatgaattaa tctgtgtgga
3421 tgggacacca gtaattggga aatcacacca gctcgtggtc cagcttatgc aacaagctgc
3481 caagcaaggc catgtcaatc tcacagtgag gcggaaagtg gtctttgccg tccccaaagc
3541 agagaatgag gtgccctcac cagcctcatc acaccacagt agcaaccagc ccgcgtccct
3601 gacggaggag aaacgcacac cgcaaggcag ccagaactct ctgaacactg tgagctctgg
3661 cagcggcagc accagtggca ttggcagtgg tggcggcggg ggcagcggtg tggtgagcgc
3721 tgtgctgcag ccctatgatg tggagattcg gcgtggggag aacgagggct ttgggtttgt
3781 catcgtgtcc tccgtgagca ggcccgaagc gggcacaacc ttcgagtcct caaatgccac
3841 gctgctgact aatgctgaga agattgccac catcaccacc actcatgccc cctctcagca
3901 ggggacccag gaaacaagga ccaccaccaa accaaagcag gattctcagt ttgagttcaa
3961 aggaccgcag gctgcacagg agcaagattt ctacactgtg gaattggaaa gaggggccaa
4021 gggatttggc tttagtcttc gagggggccg agaatataac atggatcttt atgttctgcg
4081 cttggcagag gatggtcctg cagaaagatg tgggaagatg aggattggcg atgaaattct
4141 agagatcaat ggtgagacca ccaaaaacat gaaacactct cgggccatag aactgatcaa
4201 gaatggcggc cgcagggtcc gtctgtttct gcggcgggga gacggctcag tcccagaata
4261 tgcgatgatc cctcctaaaa tcgctgcatg tatgagaaat gaaaagctcg gggaggcttg
4321 cttctacctt atgggccata atcaaactac gaccccagca gcgacaggaa cggcccctcc
4381 accggtgcac aaggtgttcc ggaagtgagg ccggggccgc cagaccacag accgcatcca
4441 gccttggagt ccagttaccc acccgaactt cacaaatcat cacaacatgc cgaaaagcga
4501 gcacacgcga aggatccaaa aggcaacagg gagcacagca aacaacccaa cgaacatcac
4561 acctggaatg gaacttctag aaaaacagga cagcggggca tgccggccca aagaccggcc
4621 acccgacgca tggagagagg cgcagccaga gcggacagcc accaatggtt caaagaggag
4681 gtcaccggag aagcgcaggg aaggcacccg cagcgctgac aacactttgg aaagaaggga
4741 gaagcatgag aagagaagag agatatctcc cgagaggaag cgagagcgtt cacccacccg
4801 gagaaaagat agctccccga gccgccggcg gaggtccctc gaaagactcc tggatcaaag
4861 acggtcccca gagcgcagaa gagggggctc ccccgagagg agagccaaat ccaccgacag
4921 gaggagggcc aggtcccctg agcgcaggcg agagcggtca ctggacaaaa ggaaccggga
4981 cgacaaggtt ggccaccgag aaagagagga ggctggtctg aagctggaag cggggagaag
5041 cccccgaaat cccccagagc agagaaggcg gccttacaaa gaatgtagca ccgacctcag
5101 catctgagac tcagcattgc aacctttacc gtgtcaaagg ttctaagagg aaggtcgcaa
5161 aacgaaatca tttagtttct tacctgagga agcatctgac acctgatagt cctaagaata
5221 gtaacgaaca ttttatgcat ctgaaatctt ataaggaaaa atatggaatt ctgcagatat
5281 ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
5341 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
5401 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
5461 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
5521 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
5581 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
5641 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
5701 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
5761 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
5821 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
5881 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
5941 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
6001 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
6061 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
6121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
6181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
6241 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
6301 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
6361 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
6421 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
6481 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
6541 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
6601 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
6661 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
6721 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
6781 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
6841 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
6901 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
6961 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
7021 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
7081 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
7141 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
7201 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
7261 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
7321 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
7381 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
7441 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
7501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
7561 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
7621 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
7681 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
7741 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
7861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
7921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
7981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
8041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
8101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
8161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
8221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
8281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
8341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
8401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
8461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
8521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
8581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
8641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
8701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
8761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
8821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
8881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
8941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
9001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
9061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
9121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
9181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
9241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
9301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
9361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
9421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
9481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
9541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
9601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
9661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
9721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
9781 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgcc
//
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